Use of DNA fingerprinting to assess genetic variability and purification of specific proteins from oilseed rape meal

Summary

Sector:
Cereals & Oilseeds
Project code:
PR328
Date:
01 July 2000 - 30 June 2003
Funders:
AHDB Cereals & Oilseeds.
AHDB sector cost:
£55,032 from HGCA (project no. 2325)
Total project value:
£950,000 from the European Union
Project leader:
N. PRICE, M. Q. CHAUDHRY, H. ATTERBY, P. JENNINGS and T. FRANKLIN Central Science Laboratory, Sand Hutton, York YO41 1LZ

Downloads

pr328-final-project-report

About this project

Abstract

The overall aim of this project was to explore the feasibility of obtaining added-value products from de-oiled oilseed rape (OSR) meal. The research and development work was carried out by 14 partners from different EU countries. The research carried out at CSL was jointly funded by the European Commission and HGCA.

As part of the project, two classes of bioactive proteins were purified and characterised at CSL. These included proteins with a potent antifungal activity (thionins), and proteins with a strong proteinase inhibitor action. A method for preparing semi-purified extracts with antifungal activity from larger quantities of OSR meal was also devised.

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