Research project reports on nematode pests of potatoes
The AHDB research projects on nematode pests of potatoes have been categorised according to the topics covered, to allow readers to identify the most relevant report(s).
Click on a project title to access more information on the project, including the final report.
Biology of potato cyst nematodes (PCN)
- national survey of occurrence of the two PCN species in GB and the G. pallida pathotypes present
- comparison of lab-based methods to test PCN viability
- rate and amount of PCN hatching according to temperature
- monitoring of field trials to understand if a second generation of PCN occurs in GB
Sampling soils for PCN
- proficiency of seven GB laboratories to assess the level of soil infestation by potato cyst nematode
- soil sampling for PCN
- lab proficiency testing
- the PCN problem in Scotland and grower interviews about their approaches to PCN management
- DNA-based diagnostics for testing soil for PCN, without the need to extract cysts from soil
Potato varieties and the management of PCN
An educational tool (PCN calculator) was developed to demonstrate how PCN populations change with factors such as the variety grown, soil type and whether nematicides are used. It requires information on variety tolerance and resistance to PCN. Projects were undertaken to generate tolerance data.
- tolerance of 10 potato varieties based on small plot trials
- tolerance of 12 potato varieties based on small plot trials
- 1 tonne boxes, with and without introduced PCN inoculum, were used to test if the system would generate consistent information on variety tolerance
- molecular techniques were used to assess the likelihood that variety resistance to G. pallida will breakdown
- combining a range of different resistance sources in a single variety will be the most durable approach
- studied the genetic basis of H2 resistance
- H2 provides resistance against G. pallida pathotype Pa1 whilst also conferring a lower level of control of pathotypes Pa2/3
- location of the H2 gene mapped to potato chromosome 5
- preliminary breeding combining resistance to G. rostochiensis (H1), G. pallida (H3) and H2 produced plants that were highly resistant to both nematodes.
Other management options
- pot-based evaluation of Solanum species as trap crops for PCN
- information on their optimum seed rates and herbicide sensitivity
- field evaluation of biofumigants for PCN management
- comparison of incorporation techniques
- impact of nitrogen and sulphur rates on biofumigant biomass and PCN numbers post-incorporation.
- seedling density and isothiocyanate-release
- rate of loss of biofumigant compound(s) after incorporation
- toxicity of volatile halide compounds to potato cyst nematode (PCN) juveniles
- sensitivity of Rhizoctonia solani and Verticillium dahliae to isothiocyanates
- decline in viability of PCN during growth of a brown mustard crop and after its incorporation
- biofumigation and free-living nematodes
- hatching profiles for PCN cysts
- efficacy of oxamyl applied as a granule or as a liquid via drip irrigation systems
- factors that affect PCN decline rates between potato crops
- techniques for measuring decline rates
- fungal parasites of PCN and their sensitivity to fungicides
- lab studies of PCN hatch in the presence of Solanum sisymbriifolium
- effects of granular nematicides or fungal pathogens on PCN multiplication.
Management of free-living plant parasitic nematodes (FLN)
- DNA-based detection of Longidorus, Paratrichodorus and Trichodorus species
- sampling guidelines for testing soil for FLN and tobacco rattle virus (TRV)
- impact of (Para)Trichodorus direct feeding damage on yield and processing quality
- testing alternative control options
- identifying molecular markers to facilitate the breeding of new potato varieties with resistance to TRV
- molecular diagnostics used to map the occurrence of TRV in the roots of weeds in fields prior to potato cropping
- distribution of free-living nematodes and TRV
- effects of TRV on potatoes
- sampling potato fields for presence of root lesion nematodes
- improved molecular diagnostics for detection of Pratylenchus
- Impact of penetrans on potato yield
- information on species of concern, their host ranges, identification and control.